Image processing problem Follow

My editor cannot access a file to train a neural network.

import os
import cv2
import numpy as np
import matplotlib.pyplot as plt
import scipy

ROWS = 64
COLS = 64
CHANNELS = 3

TRAIN_DIR = 'Train_data/'
TEST_DIR = 'Test_data/'

train_images = [TRAIN_DIR + i for i in os.listdir(TRAIN_DIR)]
test_images = [TEST_DIR + i for i in os.listdir(TEST_DIR)]

print(len(test_images))

Do I have to click some tab on pycharm to let it access the data?